ABSTRACT
Coronary artery disease [CAD] is the leading cause of mortality in many parts of the world. Genome-wide association studies [GWAS] have identified several genetic variants associated with CAD in Low-density lipoprotein receptor [LDLR] locus. This study was evaluated the possible association of genetic markers at LDLR locus with CAD irrespective to lipid profile and as well as the association of these SNPs with severity of CAD in Iranian population. Sequencing of 2 exons in LDLR gene [Exon 2, 12] and part of intron 30 of SMARCA4 gene include rs1122608, was performed in 170 Iranian patients angiographically confirmed CAD and 104 healthy controls by direct sequencing. Sullivan's scoring system was used for determining the severity of CAD in cases. Our results showed that homozygote genotypes of rs1122608 [P<0.0001], rs4300767 [P<0.005] and rs10417578 [p<0.007] SNPs have strong protective effects on the CAD. In addition, we found that rs1122608 [GT or TT] was at higher risk of three vessel involvement compared to single vessels affecting [P=0.01]
ABSTRACT
To investigate the influence of Commiphora mukul resin [guggul] on the knee articular cartilage of rats in experimental osteoarthritis. To induce osteoarthritis [OA], single intra-articular injection of MIA [1 mg] in the rat knee joints was performed. Histopathological changes in knee joints were observed after two weeks. The beneficial effect of guggul was studied, orally. The early OA characterized by areas of chondrocytes degeneration in the tibial plateaus and femorat condyles. Changes to the subchondral bone and proteog[ycane contents and synoviat cell proliferation, were observed. Administration of guggul prevented the negative effects of iodoacetate, dose dependently. Chondrocyte damages were significantly prevented and proteoglycane were less affected, especially in high guggul dose and no cell proliferation and inflammatory cell were detected in synovial. The effectiveness of guggut in improvement of histopathotogical damages is emphasized and its chondroprotective effect in vivo is highlighted